Cher will perform at Jacksonville Veterans Memorial Arena on May 14 as part of her "Dressed to Kill" tour.
The 49-city tour begins in Phoenix on March 22. She'll perform on May 16 in Orlando and May 17 in Fort Lauderdale.
Ticket prices and box office sale dates were not announced, although the promoter said American Express cardholders will be able to buy tickets beginning at 10 a.m. Monday.
Anyone who buys tickets online will receive a free copy of her "Closer to the Truth" album that was released on Tuesday.
Cher last performed in Jacksonville in 2003 during her "Farewell Tour."