More than 200 veterans -- including some Pearl Harbor survivors -- attended the remembrance ceremony aboard the USS DeWert.
The service, which has been a Mayport tradition since 1966, included several speakers and the traditional throwing of flowers over the side of the ship.
The Japanese killed 2,390 service members and 49 civilians in the attack 71 years ago Friday.
A 21-gun salute was part of the Friday's ceremony honring those who fell that day.
Part of the tradition at Mayport's Pearl Harbor remembrance is throwing flowers overboard to honor their fallen comrades.
"I'm glad to see the sons and daughters of the veterans of Pearl Harbor are carrying on the tradition, and will keep it up for the rest of time so we're very important to do that," said Florida's VFW liaison to the Department of Military Affairs.