The Florida Gators will face Connecticut in the Final Four next Saturday in Arlington, Texas at 6:09 PM.
In the other semifinal Saturday night Kentucky will meet Wisconsin 30 minutes after the first game ends.
The Huskies (30-8) beat Michigan State 60-54 in the East Regional Final on Sunday.
The Gators advanced to Saturday's game with a 62-52 win over Dayton in the South Regional Final yesterday.
UConn is the last team to beat the Gators this year. The Gators have won 30 consecutive games since that 65-64 loss on December 2, 2013 in Storrs, Connecticut.
The Gators have faced the other three teams in the final four this year.
"It definitely helps," coach Billy Donovan said Monday. "It helps both teams. There will be a familiarity there because they've been on the floor with those guys. ... It gives you at least a reference point of what you're dealing with there."
UConn head coach Kevin Ollie is looking forward to the rematch.
"We're going to be well prepared, because I know about these guys' heart," Ollie said. "That's what got us through: It was a heart of a champion, heart of a lion."
Scottie Wilbekin hurt his ankle with three minutes left and Huskies point guard Shabazz Napier hit a jumper at the buzzer for the win.